xbai restriction sites (New England Biolabs)
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xbai restriction sites
Xbai Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai+restriction+sites/XbaI/us12606611-569-10-25
Average 99 stars, based on 6874 article reviews
Xbai Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbai+restriction+sites/XbaI/us12606611-569-10-25
Average 99 stars, based on 6874 article reviews
xbai restriction sites - by Bioz Stars,
2026-09
99/100 stars
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Clone Assay:Article Title: An optimized and robust workflow for quantifying the canonical histone ubiquitination marks H2AK119ub and H2BK120ub by LC-MS/MS Article Snippet: The cDNA encoding wild-type human histone H2A type 1 (UniProt accession P0C0S8), fused to the V5 epitope tag at the N- or C-terminus, was purchased as a synthetic gene block from IDT. .. The cDNA was cloned into a derivative of a pLenti-PGK-puro vector (Addgene #19360) between the SalI and Article Title: An optimized and robust workflow for quantifying the canonical histone ubiquitination marks H2AK119ub and H2BK120ub by LC-MS/MS Article Snippet: The cDNA encoding wild-type human histone H2A type 1 (UniProt accession P0C0S8 ), fused to the V5 epitope tag at the N- or C-terminus, was purchased as a synthetic gene block from IDT. .. The cDNA was cloned into a derivative of a pLenti-PGK-puro vector (Addgene #19360) between the SalI and Sequencing:Article Title: Antibodies which specifically bind to dengue virus serotype 4 (DENV4) non-structural protein 1 (NS1) and methods of use thereof to detect a DENV4 infection Article Snippet: These primers were designed to introduce restriction sites for BmsBI and XbaI in order to clone the full length of DENV4 NS1 gene into a directional pl-secSUMO* cloning vector (LifeSensors, Malvern, PA). .. Prior to primer design, the complete absence of BmsBI and Article Title: Astrocytes amplify neurovascular coupling to sustained activation of neocortex in awake mice Article Snippet: An open-source pulse train generator (Sanworks, Pulse Pal v2) was configured to synchronize trigger signals between the cameras, LEDs, the Picospritzer III and the microscope. .. To generate the AAV astrocyte-targeted expression construct of HA-tagged CalEx, the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ; http://n2t.net/addgene:111568 ; RRID:Addgene_111568) with primers designed to incorporate a N-terminal HA tag encoding the amino acids YPYDVPDYA and used to replace mCherry-PMCA2w/b in the same backbone at the 5’ NheI and 3’ Polymerase Chain Reaction:Article Title: Phylogenetic and Complementation Analysis of a Single-Stranded DNA Binding Protein Family from Lactococcal Phages Indicates a Non-Bacterial Origin Article Snippet: .. BamHI and Article Title: Endocytosis-like DNA uptake by cell wall-deficient bacteria. Article Snippet: .. All restriction enzymes were ordered from New England Biolabs. pRK1 (Supplementary Table 5) was created by amplifying the upstream flanking region of comEA by PCR with primers FL1-comEA/ comEC-FW and FL1-comEA/comEC-REV, thereby introducing unique EcoRI and Article Title: Astrocytes amplify neurovascular coupling to sustained activation of neocortex in awake mice Article Snippet: An open-source pulse train generator (Sanworks, Pulse Pal v2) was configured to synchronize trigger signals between the cameras, LEDs, the Picospritzer III and the microscope. .. To generate the AAV astrocyte-targeted expression construct of HA-tagged CalEx, the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ; http://n2t.net/addgene:111568 ; RRID:Addgene_111568) with primers designed to incorporate a N-terminal HA tag encoding the amino acids YPYDVPDYA and used to replace mCherry-PMCA2w/b in the same backbone at the 5’ NheI and 3’ Bioprocessing:Article Title: Astrocytes amplify neurovascular coupling to sustained activation of neocortex in awake mice Article Snippet: An open-source pulse train generator (Sanworks, Pulse Pal v2) was configured to synchronize trigger signals between the cameras, LEDs, the Picospritzer III and the microscope. .. To generate the AAV astrocyte-targeted expression construct of HA-tagged CalEx, the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ; http://n2t.net/addgene:111568 ; RRID:Addgene_111568) with primers designed to incorporate a N-terminal HA tag encoding the amino acids YPYDVPDYA and used to replace mCherry-PMCA2w/b in the same backbone at the 5’ NheI and 3’ Expressing:Article Title: Astrocytes amplify neurovascular coupling to sustained activation of neocortex in awake mice Article Snippet: An open-source pulse train generator (Sanworks, Pulse Pal v2) was configured to synchronize trigger signals between the cameras, LEDs, the Picospritzer III and the microscope. .. To generate the AAV astrocyte-targeted expression construct of HA-tagged CalEx, the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ; http://n2t.net/addgene:111568 ; RRID:Addgene_111568) with primers designed to incorporate a N-terminal HA tag encoding the amino acids YPYDVPDYA and used to replace mCherry-PMCA2w/b in the same backbone at the 5’ NheI and 3’ Construct:Article Title: Astrocytes amplify neurovascular coupling to sustained activation of neocortex in awake mice Article Snippet: An open-source pulse train generator (Sanworks, Pulse Pal v2) was configured to synchronize trigger signals between the cameras, LEDs, the Picospritzer III and the microscope. .. To generate the AAV astrocyte-targeted expression construct of HA-tagged CalEx, the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ; http://n2t.net/addgene:111568 ; RRID:Addgene_111568) with primers designed to incorporate a N-terminal HA tag encoding the amino acids YPYDVPDYA and used to replace mCherry-PMCA2w/b in the same backbone at the 5’ NheI and 3’ Article Title: RNA molecules, methods of producing circular RNA, and treatment methods Article Snippet: Reactions of RNA Templates with T4 PNK and RtcB: After gel purification of autocatalytically cleaved RNA, 300 pmol were treated with T4 polynucleotide kinase (New England Biolabs M0201) according to the manufacturer's protocol at 37° C. for 30 minutes and inactivated for 20 minutes at 65° C. The products were cleaned by phenol chloroform extraction using heavy phase-lock tubes (Quantabio 2302830). .. 10 pmol of this purified T4-PNK-treated RNA or of the gel purified RNA was ligated using RtcB Ligase (New England Biolabs M0458) for 1 hour at 37° C. Cloning of Autocatalytic circRNA Constructs: DNA templates containing Broccoli and each of the ribozyme combinations were prepared as described above with flanking SalI and Amplification:Article Title: Astrocytes amplify neurovascular coupling to sustained activation of neocortex in awake mice Article Snippet: An open-source pulse train generator (Sanworks, Pulse Pal v2) was configured to synchronize trigger signals between the cameras, LEDs, the Picospritzer III and the microscope. .. To generate the AAV astrocyte-targeted expression construct of HA-tagged CalEx, the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ; http://n2t.net/addgene:111568 ; RRID:Addgene_111568) with primers designed to incorporate a N-terminal HA tag encoding the amino acids YPYDVPDYA and used to replace mCherry-PMCA2w/b in the same backbone at the 5’ NheI and 3’ Plasmid Preparation:Article Title: Astrocytes amplify neurovascular coupling to sustained activation of neocortex in awake mice Article Snippet: An open-source pulse train generator (Sanworks, Pulse Pal v2) was configured to synchronize trigger signals between the cameras, LEDs, the Picospritzer III and the microscope. .. To generate the AAV astrocyte-targeted expression construct of HA-tagged CalEx, the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ; http://n2t.net/addgene:111568 ; RRID:Addgene_111568) with primers designed to incorporate a N-terminal HA tag encoding the amino acids YPYDVPDYA and used to replace mCherry-PMCA2w/b in the same backbone at the 5’ NheI and 3’ Purification:Article Title: RNA molecules, methods of producing circular RNA, and treatment methods Article Snippet: Reactions of RNA Templates with T4 PNK and RtcB: After gel purification of autocatalytically cleaved RNA, 300 pmol were treated with T4 polynucleotide kinase (New England Biolabs M0201) according to the manufacturer's protocol at 37° C. for 30 minutes and inactivated for 20 minutes at 65° C. The products were cleaned by phenol chloroform extraction using heavy phase-lock tubes (Quantabio 2302830). .. 10 pmol of this purified T4-PNK-treated RNA or of the gel purified RNA was ligated using RtcB Ligase (New England Biolabs M0458) for 1 hour at 37° C. Cloning of Autocatalytic circRNA Constructs: DNA templates containing Broccoli and each of the ribozyme combinations were prepared as described above with flanking SalI and Cloning:Article Title: RNA molecules, methods of producing circular RNA, and treatment methods Article Snippet: Reactions of RNA Templates with T4 PNK and RtcB: After gel purification of autocatalytically cleaved RNA, 300 pmol were treated with T4 polynucleotide kinase (New England Biolabs M0201) according to the manufacturer's protocol at 37° C. for 30 minutes and inactivated for 20 minutes at 65° C. The products were cleaned by phenol chloroform extraction using heavy phase-lock tubes (Quantabio 2302830). .. 10 pmol of this purified T4-PNK-treated RNA or of the gel purified RNA was ligated using RtcB Ligase (New England Biolabs M0458) for 1 hour at 37° C. Cloning of Autocatalytic circRNA Constructs: DNA templates containing Broccoli and each of the ribozyme combinations were prepared as described above with flanking SalI and |